Journal: Pharmaceutics
Article Title: Non-Invasive, Targeted Nanoparticle-Mediated Drug Delivery across a Novel Human BBB Model
doi: 10.3390/pharmaceutics15051382
Figure Lengend Snippet: Image of Western blot membrane, detecting ZO1 TJ-protein (mw 195 kDA) in lysed cells from different in vitro BBB models. Endothelial cells (E), Pericytes (P), Astrocytes (A), Fibronectin + Gelatin (F + G), Fibronectin + Collagen (F + C), No substrata (NS) and Magnesium (Mg 2+ ), Dynamic mode.
Article Snippet: Immortalised human brain microvascular endothelial cells (I-HBMEC) (Innoprot, Bizkaia, Spain) (P10361-1M), Immortalised human astrocytes (IA) (Innoprot, Bizkaia, Spain) (P10251-1M), CLTH/Immortalised Pericytes (IP) (Amsbio, Oxfordshire, U.K.) (CL05008-CLTH), D-MEM/F:12 (1:1) (CE) (Thermofisher, Cambridge, U.K.) (11320074), Insulin-trans-sel-G, 100× (Thermofisher, Cambridge, U.K.) (41400045), Penicillin streptomycin (PS) (Gibco, Paisley, U.K.) (15070-063), Fetal bovine serum (FBS) (Gibco, Paisley, U.K.) (A3160801), Hydrocortisone solution (50 um) (Merck, Dorset, U.K.) (H6909), Fibroblast growth factor-basic (BFGF) (Merck, Dorset, U.K.) (SRP3043), Heparin sodium salt (Merck, Dorset, U.K.) (H3149), Dulbecco’s phosphate buffered saline, M (DPBS) (Merck, Dorset, U.K.) (D8537), Accutase ® solution (Merck, Dorset, U.K.) (A6964), Tissue culture flask, 75 cm 2 growth area (T75) (Sarstedt, Leicestershire, U.K.) (83.3911.002).
Techniques: Western Blot, Membrane, In Vitro